We demonstrate diagnostic imaging of ALI with NAPs show NAP tropism for inflamed human donor lungs and show that NAPs can remediate pulmonary oedema in ALI. NAP uptake in neutrophils was shown to depend on complement opsonization. Protein-conjugated liposomes were engineered to recapitulate NAP tropism for pulmonary neutrophils. Flow cytometry and immunohistochemistry showed NAPs have tropism for pulmonary neutrophils.
Nanoparticles with symmetric protein arrangement (for example, viral capsids) had no selectivity for inflamed lungs. We observed homing to inflamed lungs for a variety of nanoparticles with agglutinated protein (NAPs), defined by arrangement of protein in or on the nanoparticles via hydrophobic interactions, crosslinking and electrostatic interactions. This study shows that the supramolecular arrangement of proteins in nanoparticle structures predicts nanoparticle accumulation in neutrophils in acute lung inflammation (ALI). Nature Nanotechnology volume 17, pages 86–97 ( 2022) Cite this article Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation
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